Abstract
Sixty-five children aged 1 month to 15 years with a diagnosis of viral encephalitis, who had been admitted to the Clinic of the Pediatric Research and Clinical Center for Infectious Diseases, Federal Biomedical Agency, in 2013-2016 were examined. Objective: to establish the frequency of mixed herpesvirus infection in children with viral encephalitis, by using the current diagnostic methods to determine the role of reactivation of herpesvirus infection on the development of viral encephalitis. The investigators identified anti-herpesvirus antibodies and their avidity using an enzyme immunoassay and anti-cytomegalovirus antibodies specific for antigens by immunoblotting applying the kits. Herpesvirus DNA was detected in the biological material (blood, cerebrospinal fluid) by real-time polymerase chain reaction (PCR) and by electrophoresis using the PCR kits (Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Welfare (Moscow). Young children were shown to develop the disease as a result of primary infection more commonly as monoinfection in the old age groups during the persistence of one or two herpesviruses in the body. In encephalitis caused by herpes simplex virus type 1 and type 2, reactivation of cytomegalovirus that could be a cofactor in the development of brain inflammation was proven by the current diagnostic technique immunoblotting. The comprehensive laboratory approach to the etiological diagnosis of viral encephalitis, which encompassesthe determination of antibody classes, avidity, and antibodiesto individual protein determinants of viral particles, makes it possible to specify the activity of different herpesviruses in mixed infection and to determine the stage of the infectious process, which is important for the choice of therapeutic policy.
Highlights
Sixty-five children aged 1 month to 15 years with a diagnosis of viral encephalitis, who had been admitted to the Clinic of the Pediatric Research and Clinical Center for Infectious Diseases, Federal Biomedical Agency, in 2013-2016 were examined
Objective: to establish the frequency of mixed herpesvirus infection in children with viral encephalitis, by using the current diagnostic methods to determine the role of reactivation of herpesvirus infection on the development of viral encephalitis
The investigators identified anti-herpesvirus antibodies and their avidity using an enzyme immunoassay and anti-cytomegalovirus antibodies specific for antigens by immunoblotting applying the kits
Summary
Современная диагностика микстгерпесвирусной инфекции у детей с вирусными энцефалитами. Current diagnosis of mixed herpesvirus infection in children with viral encephalitis. Цель работы: установить частоту смешанного герпесвирусного инфицирования при вирусных энцефалитах у детей и с применением современных методов диагностики определить роль реактивации герпесвирусной инфекции в развитии вирусных энцефалитов. Для цитирования: Голева О.В., Мурина Е.А., Скрипченко Н.В., Имянитов Е.Н., Иванова Р.А. For citation: Goleva O.V., Murina E.A., Skripchenko N.V., Imyanitov E.N., Ivanova R.A. Current diagnosis of mixed herpesvirus infection in children with viral encephalitis. В настоящее время отсутствует достаточная информация о степени участия персистирующих герпесвирусов в развитии демиелинизирующих процессов в тканях головного мозга на фоне острого инфекционного заболевания, вызванного другими вирусными агентами [8]. Цель работы: установить частоту встречаемости смешанного герпесвирусного инфицирования при вирусных энцефалитах у детей и с применением современных методов диагностики определить степень влияния реактивации герпесвирусной инфекции на развитие вирусных энцефалитов
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