Abstract

In most woody fruit species, transformation and regeneration are difficult. However, European plum (Prunus domestica) has been shown to be amenable to genetic improvement technologies from classical hybridization, to genetic engineering, to rapid cycle crop breeding (‘FasTrack’ breeding). Since the first report on European plum transformation with marker genes in the early 90 s, numerous manuscripts have been published reporting the generation of new clones with agronomically interesting traits, such as pests, diseases and/or abiotic stress resistance, shorter juvenile period, dwarfing, continuous flowering, etc. This review focuses on the main advances in genetic transformation of European plum achieved to date, and the lines of work that are converting genetic engineering into a contemporary breeding tool for this species.

Highlights

  • European plum breeding objectivesPlum scion breeding programs are established by the market requirements and consumer demand for the fruit as well as the regional climatic conditions, soils, and pest/disease pressures

  • This review focuses on the main advances in genetic transformation of European plum achieved to date, and the lines of work that are converting genetic engineering into a contemporary breeding tool for this species

  • This review focuses on the main advances in genetic transformation of European plum achieved to date, and the lines of work that are Transgenic Res (2018) 27:225–240 converting genetic engineering into a contemporary breeding tool for this species

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Summary

European plum breeding objectives

Plum scion breeding programs are established by the market requirements and consumer demand for the fruit as well as the regional climatic conditions, soils, and pest/disease pressures. Main breeding goals include resistance to biotic and/or abiotic stress, chilling requirements, tree size, productivity and fruit quality traits (Callahan 2008; Neumuller 2011). Related to plum affecting diseases breeding efforts have been focused on: brown rot, caused by the fungus Monilinia spp.; bacterial canker, caused by Pseudomonas syringae van Hall; bacterial spot, caused by Xanthomonas campestris pv. One of the major pests in stone fruit orchards worldwide are Root-knot nematode (RKN), obtaining resistant rootstocks is a main goal. There are not yet molecular markers for agronomic traits available in Prunus domestica, which could be applied in breeding programs, due to the highly polymorphic hexaploid genome of this species (Neumuller 2011). In rootstock breeding programs MAS is being routinely used for selection of root-knot nematode (RKN) resistance since years ago (Claverie et al 2004; Dirlewanger et al 2004; Lecouls et al 2004)

Genetic transformation of European plum
Claudia verde
Leaves Hypocotyls
Plum pox virus resistance
Fruit softening delayed plums
Soil pathogens resistance
Abiotic stress resistance
Modified size and shape trees
Gene editing
Findings
Conclusions

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