Abstract
Ubiquitous Pseudomonads have great potential to influence the speciation and mobility of actinides in the environment. This study explores the unknown interaction between curium(III) and cell-suspensions of Pseudomonas fluorescens (CCUG 32456) isolated from the Äspö site, Sweden. The interaction between curium(III) and P. fluorescens cells was studied at trace curium(III) concentrations (0.3 μM) using time-resolved laser-induced fluorescence spectroscopy. Extraction studies have shown that the biosorption of curium(III) is a reversible process. Two Cm3+−P. fluorescens (CCUG 32456) species were identified, R−O−PO3H−Cm2+ and R−COO−Cm2+, having emission maxima at 599.6 and 601.9 nm, respectively. The corresponding surface complexation constants were determined to be log β111 = 12.7 ± 0.6 and log β110 = 6.1 ± 0.5, respectively.
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