類薑黃素(curcuminoids) 與Seprafilm®在小鼠傷口癒合模式之研究

Abstract

Wound healing is a complex biological process. Wound healing consists of an orderly progression of events that re-establish the integrity of the damaged tissue, including inflammation, cell migration, angiogenesis, extracellular matrix synthesis, collagen deposition and re-epithelialization. There are three major stages in the wound healing process; the first stage is the inflammatory response, then proliferation and finally tissue regeneration. The present investigation was undertaken to determine the role of curcuminoids and Seprafilm on changes in collagen characteristics and antioxidant property during cutaneous wound healing .Turmeric (Curcuminoids), an herb isolated from the rhizome of the plant, called Curcuma Longa. Interest in this herb has grown in recent years because of its putative beneficial health effects, including antioxidant, anti-inflammatory and cancer chemopreventive actions. The curcuminoids products contain a mixture of Curcumin, the primary ingredient, with minor components of demethoxycurcumin (DMC) and bisdemethoxycurcumin (bDMC). Seprafilmr Adhesion Barrier serves as a temporary bioresorbable barrier separating apposing tissue surfaces. The physical presence of the membrane separates adhesiogenic tissue while the normal tissue repair process takes place. The wound repair process have focused on signal molecules such as cytokines and growth factors. It is well known that these factors bind to specific receptors on the membrane of fibroblasts, which initiates a signal cascade, leading to specific cellular functions such as cell locomotion. The transforming growth factor-β (TGF-β) also promotes the fibroblast proliferation and collagen deposition. The heat shock protein 47 (HSP-47) is a collagen type I that has been shown to play a major role during the processing and/or secretion of procollagen. In this study, the curcuminoids were dissolved in 95% ethanol and the ICR mice were treated daily for 6 days at dosages (0.1 and 0.5 mg/kg). Initially, various doses of curcuminoids were administered topically daily in ICR mice to evaluate its wound healing capacity. On the 7th day post wounding, the ICR mice were sacrificed and protein expressions of Inducible Nitric oxide synthase (iNOS), transforming growth factor-β, heat shock protein 47, collagen I were determined by western blotting. The MMPs expressions of MMP-2 and MMP-9 were determined by zymography (Gelatin-substrate Zymography). Our results suggest that curcuminoids was found to improve all the phases of wound repair, including collagen synthesis.