Curcumin targets miR-1297 to promote mitochondrial apoptosis and cisplatin sensitization of lung cancer stem cells

Abstract

The medicinal value of curcumin has been confirmed in many diseases. This study explores miR-1297’s role in lung cancer cells. The targeting regulation between miR-1297 and BH3-only was analyzed by luciferase activity. Cisplatin (DDP)-resistant cell lines A549/DDP and NCI-H460/DDP were established followed by analysis of cell proliferation activity, BH3-only expression, and cell proliferation by EdU staining. miR-1297 targets BH3-only. miR-1297 expression in A549/DDP and NCI-H460/DDP cells was significantly increased while BH3-only was reduced. Same dose of DDP inhibited the proliferation of A549/DDP and NCI-H460/DDP cells. BH3-only in A549/DDP and NCI-H460/DDP cells in miR-1297 inhibitor transfection group was significantly increased, apoptosis was increased, proliferation was weakened, and drug resistance was reduced. Lung cancer DDP resistance is related to the expression of miR-1297. Down-regulation of miR-1297 can reduce the DDP drug resistance by increasing the expression of BH3-only.