Curcumin Protects Human Umbilical Vein Endothelial Cells against H2O2-Induced Cell Injury.

Jipeng Ouyang,Rong Li,Haiqin Shi,Jianping Zhong

doi:10.1155/2019/3173149

Abstract

Migraine is a prevalent neurological disorder which causes a huge economic burden on society. It is thought to be a neurovascular disease with oxidative stress might be involved. Curcumin, one of the major ingredients of turmeric, has potent antioxidative and anti-inflammatory properties, but whether it could be used as a potential treatment for migraine remains to be explored. In the present study, human umbilical vein endothelial cells (HUVECs) were pretreated with various concentrations of curcumin (0 μM, 10 μM, 20 μM, 30 μM, 40 μM, and 50 μM) for 12 h, thereby exposed to H2O2 (100 μM) for another 12 h. The viability of HUVECs was tested by the CCK-8 assay, and the activities of antioxidant enzymes including superoxide dismutase (SOD) and glutathione (GSH) were also examined. Intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) were assayed to determine H2O2-induced oxidative stress. In addition, several cell death-related genes (p53, p21, Bax, and Bcl-2) were detected by PCR, and an apoptosis-related protein (caspase3) was evaluated by western blotting. Our results showed that curcumin improved the H2O2-induced decrease of cell viability and antioxidative enzyme activities and decreased the level of oxidative stress. As a conclusion, curcumin could mitigate H2O2-induced oxidative stress and cell death in HUVECs and may be a potential therapeutic drug for migraine.

Highlights

Migraine is a widespread neurological disorder with the typical clinical symptom being recurrent headache [1]. e global prevalence of migraine was reported to be 15%, and 10% will progress to chronic migraine [2]
To examine the cytotoxicity of curcumin and H2O2 on human umbilical vein endothelial cells (HUVECs), cell viability was detected by the CCK8 assay
Curcumin treatment at the concentrations of 0 μM, 10 μM, 20 μM, 30 μM, 40 μM, and 50 μM showed no cytotoxicity on HUVECs when compared with the control group (p > 0.05). erefore, the concentrations of 0 μM, 10 μM, 20 μM, 30 μM, 40 μM, 50 μM, and 100 μM were chosen for curcumin and H2O2, respectively, for the subsequent experiments

Summary

Introduction

Migraine is a widespread neurological disorder with the typical clinical symptom being recurrent headache [1]. e global prevalence of migraine was reported to be 15%, and 10% will progress to chronic migraine [2]. Migraine is a widespread neurological disorder with the typical clinical symptom being recurrent headache [1]. Long-term use of migraine drugs may cause some adverse events, such as abuse, addiction, and dependence [4]. One of the major ingredients of turmeric, attracted much attention because of its antioxidative, anticarcinogenic, antitumor, and anti-inflammatory properties [5, 6]. A growing body of evidences reports curcumin may have a beneficial antioxidative and neuroprotective potential for neurological diseases such as Alzheimer’s disease and Parkinson’s disease [7]. As reviewed by Shameemah, 17 studies have revealed the protective effect of curcumin in different cellular models of neurodegenerative disorders [8]. To the best of our knowledge, curcumin’s treatment potential in migraine has not yet been evaluated

Methods

Results

Conclusion