Curcumin-loaded a-lactalbumin nanoparticles neuroprotective effects against permethrine-induced neurotoxicity

Abstract

Curcumin, as is well known, plays the role of an antioxidant, anti-inflammatory and neuroprotective ingredient. Permethrine is a synthetic type I pyrethroidal neurotoxic pesticide that has been involved in causing neurotoxicity in dopaminergic cells and is currently the subject of extensive study in the fight against Parkinson's disease (PD). In the current study, curcumin loaded α-lactalbumin (α-LA) nanoparticles prepared by desolvation technique were used to protect dopaminergic cell line SH-SY5Y against permethrine induced neurotoxicity. These curcumin-loaded nanoparticles, according to transmission electron microscopy, and dynamic light scattering analysis, have the diameters of 100–150 nm, respectively. The encapsulation efficiency was 60.23 % ±3.1 %.The cellular uptake of curcumin was demonstrated to be mediated by these nanoparticles by spectrofluorimetric and Confocal imaging investigations. The α-lactalbumin nanoparticles loaded with curcumin exhibited greater intracellular drug absorption, extended retention, and neuroprotection compared to their corresponding curcumin concentrations. Neuroprotective effect was established by viability experiments and ROS level measurement. Furthermore, in permethrine-induced PD-like features, a-synuclein expression rose and tyrosine hydroxylase expression fell. To further shield dopaminergic neurons from neurotoxicity, curcumin-loaded lactalbumin nanoparticles would be a powerful therapeutic delivery vehicle.