Abstract
BackgroundCurcumin, one of the promising candidates for supplementary therapy in cancer treatment, has been demonstrated by numerous preclinical and clinical evidence to be beneficial in treating various cancers. Apart from the critical role in a deluge of pathological processes, some mRNAs, in particular, microRNAs (miRNAs), are also involved in the anti-tumor activity. Therefore, our research focused on the possible effects of curcumin on small cell lung cancer (SCLC) cells and drew a comprehensive transcriptomes profile by high throughput sequencing to understand the molecular mechanism of curcumin as an anti-tumor agent.MethodsFirst, we calculated the apoptosis rate of H446 cells (a human SCLC cell line) cultured with curcumin. The high output sequencing uncovered the altered expression profile of genes and miRNAs. KEGG analysis selected the potential signal pathway associated with the antiproliferative property of curcumin. Finally, miRNAs significantly changed, as well as the regulatory roles of those miRNAs in cell apoptosis were determined.ResultThe apoptosis rate of H446 cells increased under the elevated concentration of curcumin treatment. And cell cycle-related genes downregulated in the curcumin-treated cells. Besides, miRNA-548ah-5p of a high level acted as a negative role in the anticarcinogenic activity of curcumin.ConclusionOur findings not only enriched the understanding of anti-tumor activity initiated by curcumin through figuring out the downregulated cell cycle-related pathways but also shed light on its novel therapeutic application.
Highlights
Small cell lung cancer (SCLC) is one type of lung cancer, which accounts for less than 20% of all lung cancer cases diagnosed (Govindan et al, 2006)
H446 cells were examined by Annexin-V/PI apoptosis detection kit after curcumin treatment at different time points to explore its effect of curcumin on cell apoptosis
The result showed that Curcumin treatment reduced the expression of Bcl-2 and increased the expression of Bax and cytochrome-C, which are involved in crucial regulation of cell growth (Ow et al, 2008)
Summary
Small cell lung cancer (SCLC) is one type of lung cancer, which accounts for less than 20% of all lung cancer cases diagnosed (Govindan et al, 2006). Polyphenols, the most abundant organic compounds derived from a variety of plants and fruits, consist of various bioactive phytochemicals relevant to lower incidence of cardiovascular diseases, metabolic diseases, and cancer (Corrêa and Rogero, 2019). Numerous preclinical and clinical trials have validated its role in a variety of human chronic diseases: inflammation, metabolic disorders, neurological, cardiovascular, infectious, skin diseases, and cancer (Kunnumakkara et al, 2017; Tomeh et al, 2019). Our research focused on the possible effects of curcumin on small cell lung cancer (SCLC) cells and drew a comprehensive transcriptomes profile by high throughput sequencing to understand the molecular mechanism of curcumin as an anti-tumor agent
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