Abstract
Objective To observe the clinical effect of curcumin chitosan microsphere (CCM) on ulcerative colitis. Methods: Our group selected 75 cases with ulcerative colitis (UC) receiving treatment at The First Affiliated Hospital of Nanchang University as observation group, and 75 cases that were healthy and received examination at the same time as control group. We detected serum miR-224-3p, TLR4, TNF-α and NF-κB levels using the double antibody sandwich ELISA (DAS-ELISA). In animal experiments, our team applied DSS to induce IBD mice models, allocated into control group, model group, sulfasalazine group, curcumin group and CCM high dose group, CCM medium dose group, and CCM low dose group, in total 7 groups (n = 10). After molding, on 3rd day our team began intragastric administration for 10 days. On 14th d, we sacrificed the mice, conducted HE staining, observed changes in the pathological form of bowel tissue in each group, and gave inflammation scores. Taking the colon tissue and serum, our team applied ELISA to detect inflammatory factors such as TNF-α, TLR4, IFN-γ levels in supernate of tissue homogenate, as well as performed western blot to detect SDF-1, CXCR4, miR-224-3p protein expression levels in intestinal tissue. Results: TNF-α, TLR4, NF-κB expressions in observation group were signally elevated while miR-224-3p expression visually decreased. In control group, TNF-α, TLR4, NF-κB expressions in observation group were signally decreased while miR-224-3p expression visually elevated, the difference was significant (P < 0.05). After treatment, serum TNF-α, TLR4, NF-κB expressions in sulfasalazine group, curcumin group, CCM low, medium and high dose groups were signally reduced, while IFN-γ expression was elevated significantly, when comparing with those in model control group, the difference was significant (P < 0.01). Compared CCM low, medium and high dose groups with sulfasalazine group, there was a significant difference in efficacy (P < 0.05). Compared CCM low, medium and high dose groups with curcumin group, there was a significant difference in efficacy (P < 0.05). We applied western blot to detect SDF-1, CXCR4, and miR-224-3p protein expression levels, finding that CCM enhanced in SDF-1, CXCR4, and miR-224-3p protein expression levels, with significant differences when comparing with those in model control and curcumin groups. Conclusion: CCM may elevate IFN-γ level and enhance SDF-1, CXCR4, and miR-224-3p protein expression levels through inhibiting TNF-α, NF-κB, and TLR4 expressions, thus reducing inflammatory response as well as damage to colon tissue in mice with UC through anti-inflammatory effects.
Highlights
Inflammatory bowel disease (IBD) is a type of recurrent chronic nonspecific intestinal inflammatory disease, like ulcerative colitis (UC) and Crohn’s disease (CD) (Sairenji et al, 2017; Flynn & Eisenstein, 2019; Zhang & Li, 2014)
After 2-to-4-week-treatment, comparing sulfasalazine group, curcumin group and curcumin chitosan microsphere (CCM) low, medium and high dose groups, the colonic mucosal morphology injury scores were signally reduced with a significant difference (P < 0.01) compared with the model control group
TLR4 signaling pathway is transduced by a signal mediated by TLR4, and it is available to induce the activity of many rapid reaction genes, producing many effector molecules, which will participate in the body’s defense response amid IBD pathogenesis (Dejban et al, 2021; Chen et al, 2019; Wang et al, 2020; Liu et al, 2020a)
Summary
Inflammatory bowel disease (IBD) is a type of recurrent chronic nonspecific intestinal inflammatory disease, like ulcerative colitis (UC) and Crohn’s disease (CD) (Sairenji et al, 2017; Flynn & Eisenstein, 2019; Zhang & Li, 2014). With residents’ lifestyle and eating habits changing, IBD incidence and canceration ratio in China has been increasing year by year, which has become the second killer of the intestine after colorectal cancer, and has been covered by chronic diseases of urban resident basic medical insurance, which seriously affects the health and safety of patients (Malik, 2015; Wright et al, 2018; Taleban et al, 2015). It is very essential for IBD prevention and treatment to actively probe into the action target as well as treatment method of IBD. Combined with pre-work, our team applied CCM to intervene in IBD, systematically observed CCM repair impact on IBD, and explored CCM mechanism in preventing and controlling IBD from its impact on TLR4, TNF-α, IL-1β, IFN-γ, NF-κB expressions in UC rats
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