Curcumin attenuates nephrotoxicity induced by zinc oxide nanoparticles in rats.

Abstract

Curcumin (Cur) effects on renal injury induced by zinc oxide nanoparticles (NZnO) in rats were investigated. NZnO at a dose of 50mg/kg for 14days was administered to rats as intoxicated group. In protection group, Cur at a dose of 200mg/kg was administered for 7days prior to NZnO treatment and followed by concomitant administration of NZnO for 14days. Plasma concentrations of uric acid, creatinine (Cr), and blood urea nitrogen (BUN) were detected to evaluate renal injury. Malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPx) levels were determined for evaluation oxidative stress. TUNEL staining and histological changes were also performed. Administration of NZnO caused a significant elevation in the uric acid, Cr, and BUN levels. Oxidative stress was increased in the kidney by NZnO through enhancing MDA contents and reducing activities of SOD and GPx enzymes. According to histological examinations, treatment with NZnO caused proximal tubule damages, which was accompanied by the accumulation of red blood cells, infiltration of inflammatory cells, and reducing glomerular diameters. Significant increase was observed in the apoptotic index of the renal tubules in NZnO-treated rats. In present work, pretreatment of Cur reduced the histological changes, decreased biomarker levels, attenuated apoptotic index, and ameliorated oxidative stress by decreasing the MDA contents and increasing the activities of SOD and GPx enzymes. These findings indicate that Cur effectively protects against NZnO-induced nephrotoxicity in the rats.