Abstract
The purpose of this study is to determine if a preventive treatment with curcumin can protect intestinal epithelial cells from inflammatory damage induced by IFNγ. To achieve this goal we have used a human intestinal epithelial cell line (HT29) treated with IFNγ to undergo apoptotic changes that can reproduce the damage of intestinal epithelia exposed to inflammatory cytokines. In this model, we measured the effect of curcumin (curcuminoid from Curcuma Longa) added as a pre-treatment at different time intervals before stimulation with IFNγ. Curcumin administration to HT29 culture before the inflammatory stimulus IFNγ reduced the cell apoptosis rate. This effect gradually declined with the reduction of the curcumin pre-incubation time. This anti-apoptotic action by curcumin pre-treatment was paralleled by a reduction of secreted IL7 in the HT29 culture media, while there was no relevant change in the other cytokine levels. Even though curcumin pre-administration did not impact the activation of the NF-κB pathway, a slight effect on the phosphorylation of proteins in this inflammatory signaling pathway was observed. In conclusion, curcumin pre-treatment can protect intestinal cells from inflammatory damage. These results can be the basis for studying the preventive role of curcumin in inflammatory bowel diseases.
Highlights
Chronic inflammation in inflammatory bowel disease (IBD) results from an inappropriate response to intestinal microbes in a genetically-susceptible host [1].The incidence of IBD is increasing worldwide, and this increase seems to be more rapid in areas of the world that are experiencing a rapid socioeconomic transformation, from low-income, rural to high-income, urbanized countries [2]
Apoptosis rate of HT29 was assessed by the Annexin V-FITC (An) Apoptosis Detection Kit
Results are expressed as means ± standard deviation (SD), in percentage (%), normalized to the control to the control condition (Untr)
Summary
The incidence of IBD is increasing worldwide, and this increase seems to be more rapid in areas of the world that are experiencing a rapid socioeconomic transformation, from low-income, rural to high-income, urbanized countries [2]. This epidemiological data, together with the observation that children of immigrants moving from countries with a low incidence of IBD to countries with high incidence have the same risk of developing IBD as the population of the country of immigration, indicate that environmental factors may play a major role in determining the risk of the disease [3]. Exclusive enteral nutrition (EEN) with elemental and semi-elemental formulas are the most widely studied restrictive diet which, in clinical trials in children with CD, proved to reduce clinical disease activity and improve mucosal inflammation [5]
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