Abstract
Simple SummaryLike many plants in the family of Zingiberaceae, Curcuma amarissima has been traditionally used to induce healing and tissue regeneration. However, there is no scientific evidence to explain how Curcuma amarissima works to accelerate wound healing. Our data clearly proved that Curcuma amarissima extract could potentially accelerate the closure of scratch wounds of human keratinocytes by stimulating cell proliferation. The potential mechanisms underlying these effects were defined to be associated with the activated signal transduction pathways relevant to cell proliferation and survival. This strongly suggests the ability of Curcuma amarissima to enhance the process of keratinocyte reepithelization during wound healing. Our current study provides convincing evidence that supports the possibility to develop an effective wound-healing promoting agent from this plant.Many medicinal plants have been used to treat wounds. Here, we revealed the potential wound healing effects of Curcuma amarissima (CA). Our cell viability assay showed that CA extract increased the viability of HaCaT cells that were cultured in the absence of serum. This increase in cell viability was proved to be associated with the pharmacological activities of CA extract in inducing cell proliferation. To further define possible molecular mechanisms of action, we performed Western blot analysis and immunofluorescence study, and our data demonstrated that CA extract rapidly induced ERK1/2 and Akt activation. Consistently, CA extract accelerated cell migration, resulting in rapid healing of wounded human keratinocyte monolayer. Specifically, the CA-induced increase of cell monolayer wound healing was blocked by the MEK inhibitor (U0126) or the PI3K inhibitor (LY294002). Moreover, CA extract induced the expression of Mcl-1, which is an anti-apoptotic protein, supporting that CA extract enhances human keratinocyte survival. Taken together, our study provided convincing evidence that Curcuma amarissima can promote proliferation and survival of human keratinocyte through stimulating the MAPK and PI3K/Akt signaling cascades. These promising data emphasize the possibility to develop this plant as a wound healing agent for the potential application in regenerative medicine.
Highlights
Ineffective skin wound healing is becoming a big problem in the public health sector.Several factors including aging, diabetes, infection, immunodeficiency, and cancers can lead to unsuccessful wound treatment and eventually cause morbidity and mortality [1,2,3,4,5]. the human body has a great protective skin barrier, sometimes an unexpected injury is unavoidable
Curcuma Amarissima (CA) Extract Enhances Cell Viability of HaCaT Cells fingerprint analysi the ethanolic extract from Curcuma amarissima (CA) by high-performance liquid chrom
Western blot analwhich its expression is regulated by the PI3K/Akt pathways, we found that CA extract ysis and of provided more information on the intracellular location of Mcl-1, which was induced the expression this protein in a concentration-dependent manner
Summary
Ineffective skin wound healing is becoming a big problem in the public health sector.Several factors including aging, diabetes, infection, immunodeficiency, and cancers can lead to unsuccessful wound treatment and eventually cause morbidity and mortality [1,2,3,4,5]. the human body has a great protective skin barrier, sometimes an unexpected injury is unavoidable. Diabetes, infection, immunodeficiency, and cancers can lead to unsuccessful wound treatment and eventually cause morbidity and mortality [1,2,3,4,5]. A process of organisms, namely “wound healing”, is responsible for the reconstruction of injured skin. This physiological repairing system requires four stages, which include hemostasis (blood clotting), inflammation, proliferation (growth of new tissue), and maturation (remodeling) [6,7]. The most important cell types responsible for re-epithelialization are keratinocytes, which proliferate, differentiate, and migrate to heal the open wound [9]. Certain growth factors such as EGF or PDGF are approved to be used to stimulate the skin healing process [17,18,19]
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