CURATIVE EFFECTS OF ETHANOLIC EXTRACT OF Palisota Hirsuta ON NEPHROTOXICITY INDUCED BY CARBON TETRACHLORIDE

Abstract

This work was done to evaluate the effect of ethanolic extract of Palisota hirsuta on nephrotoxicity induced by carbontetrachloride (CCl4). Biochemical analyses were carried out to determine the levels of creatinine, urea, total protein, chloride, carbonate using assay kits (products of Randox Laboratories Crumlin and Teco Diagnostics), sodium, potassium (method of Tietz,) and malondialdelyde (MDA) level (thiobarbituric acid assay) and the activities of catalase and superoxide dismutase (SOD). Consequently, twenty-five albino rats were divided into 5 groups of 5 rats each, the animals were fed rats mash and water ad libitum. Group 1 (positive control) rats were administered only 0.5 ml/kg body weight of groundnut oil while groups 2, 3, 4 and 5 were induced with 0.5 ml/kg CCl4 and groundnut oil (1:1).The plant extracts were not administered to groups 1 and 2; while groups 3, 4 and 5 were administered 200, 400 and 800 mg/kg body weight of extract respectively. Results showed increased levels of creatinine, urea, chloride, carbonate and malondialdelyde levels and reduced levels of total protein and SOD activity in the group induced with CCl4, but not given the drug extract (negative control). These effects were ameliorated at different dose levels of administration of the drug extract. This work therefore demonstrates the protective effect of P.hirsuta against nephrotoxicity induced by CCL4.