CUMULUS CELL GENES AS POTENTIAL BIOMARKERS OF OOCYTE AND EMBRYO DEVELOPMENTAL COMPETENCE.

Abstract

Bogomoletz Institute of Physiology NASU, Ukraine, Kiev. The selection of embryos with high implantation potential is the most important task in assisted reproductive technology. Today, this selection is based on subjective morphological criteria such as growth rate, early cleavage, the degree of fragmentation, blastocyst formation. However, the morphological assessment alone does not accurately predict oocyte/early stage embryo competence. Thus, the development of an objective, accurate, fast and affordable tests to determine oocyte quality and embryo viability could increase the chance of a successful pregnancy and reduce the number of embryos to transfer. The advent of new technologies, the so-called OMIKS, has allowed to identify novel biomarkers that can be used in cycle of in vitro fertilization (IVF) for oocyte and / or embryo selection. During folliculogenesis oocyte plays a dominant role in regulation of cumulus (CC) and granulosa cell (GC) functions, and it is consequently believed that functions of GC and CC indirectly reflect oocyte’s competence. Cell functions and active cell processes are regulated through gene expression therefore, gene expression analysis in GC and/or CC could provide a non-invasive method for identification of the most competent oocytes and embryos. In cumulus cells, genes have been identified that characterize the oocyte ability to undergo meiotic maturation, successful fertilization and early embryonic development. Among them cyclooxygenase 2, gremlin 1 and hyaluronan synthase-2, which play an important roles during oocyte development, ovulation and fertilization. This article reviews the recent data regarding these genes as potential biomarkers for selection of oocytes and embryos in the IVF program.