Culturing primitive hemopoietic cells. Long-term mouse marrow cultures and the establishment of factor-dependent (FDCP-Mix) hemopoietic cell lines.

Abstract

The maintenance of normal primitive hemopoietic cell types in culture for long periods can be achieved either by culture of hemopoietic cells in association with bone marrow-derived stromal cells, as in long-term cultures, or by establishing factor-dependent primitive hemopoietic cell lines (FDCP-Mix cell lines). The long-term culture technique and a reproducible method for establishing FDCP-Mix cell lines will be described here (1,2).