Abstract
Nowadays, the water pollution is leading issue due to palm oil mill effluent (POME), waste from palm oil production is a big problem to Malaysia which is largest palm oil producers. POME containing large amount of chemical oxygen demand (COD) which can cause severe pollution to the environment especially water. This present study designed for culturing green algae (Chlorella sp.) isolated from Pantai Balok, Kuantan which is most common microalgae with palm oil mill effluent (POME). Chlorella sp. was cultured using BG-11 medium with the addition of POME as experiment and without POME as control. The POME responsible for the growth of green algae is studied by measurement of the growth rate, total cell count and chemical oxygen demand (COD) for both conditions. The composition for control and experiment is also measured and determined by using Gas Chromatography Mass Spectrometry (GCMS). From the results, it is observed that the total cell count and growth rate of Chlorella sp. greater in presence of POME since the green microalgae absorbs the essential nutrients from the POME as their nutrients. GCMS revealed that the difference in composition for both the conditions. Results concluded that several COD also vigorously deplete with the help of green algae digestion. Cultivation of Chlorella sp. in POME will help in future water pollution treatment.
Highlights
The algae are found as the diverse group of photosynthetic organisms
The microalgae that have been collected from Teluk Cempedak that were isolated were observed under fluorescent microscope to identify according the microalgae manual book
In the present study wastewater from palm oil industries namely palm oil mill effluent (POME) must be discharged well and with a good treatment because it can lead to a water pollution that will affect an aquatic organism
Summary
The algae are found as the diverse group of photosynthetic organisms. This organism uses photons from the sunlight and chlorophyll (green pigment in algae) to prepare their own food. As a result of photosynthetic activities, algae produce a large portion of oxygen present in this atmosphere and generate huge amount of organic carbon (Bhuyar et al, 2019a). They are microscopic and macroscopic, unicellular or multicellular, mobile and immobile, attached and free-living (Tipnee et al, 2015). The algae can be found in water such as river, sea and estuary at different geographic latitudes They grow in water with different level of salinity, organic matter, hydrogen ions and at variety of temperature. Microalgae, a type of algae are one of the ancient microorganisms on earth They are single cell organisms, including of both bacteria and eukaryotes. Common microalgae are including in Eukaryotes which have organelles to control the functions of the cell, for the purpose of survive and reproduce (Ramaraj et al, 2014a)
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