Abstract
T51B rat liver cells in the exponential phase of growth were arrested in late G1 by medium calcium deprivation, exposed to mouse monoclonal anti-cytokeratin (Mr = 55,000) by addition of the antibody to the medium, induced to enter the S phase by readdition of 1.5 mM calcium, and incubated for a further 18 h. After fixing and staining with FITC-conjugated anti-mouse IgG, the cells exhibited an intact intracellular cytokeratin-staining pattern. No effect of antibody was observed on entry of cells into the S phase. These results show that rat liver-derived epithelial cells can actively take up macromolecules like cytokeratin antibodies without microinjection.
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