Abstract
Progress in the development of systems for murine oocyte growth and maturation in vitro is described. Fully grown germinal vesicle stage oocytes undergo spontaneous meiotic maturation when liberated from Graafian follicles and cultured in appropriate medium. When compared to ova induced to mature in vivo by the administration of exogeneous gonadotropins, equivalent percentages of spontaneously matured ova were fertilized and developed to live offspring. The presence of a high molecular weight component of serum was found to be essential during oocyte maturation in culture in order for the ova to become fertilizable. All of the sera tested contained similar activity except for human cord serum. None of the hormones or growth factors that were tested could substitute for serum. A higher percentage of ova became fertilizable when the cumulus cells were present during oocyte maturation.
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