Abstract
BackgroundIn a previous study, we demonstrated that intravenous administration of adipose tissue stem cells (ASCs) could significantly reduce allergic symptoms and suppress eosinophilic inflammation.MethodsTo evaluate the secretome of ASCs, we administrated culture supernatant of ASCs (ASC sup, which contains the ASC secretome) and uncultured fresh medium (con sup) into a mouse model of allergic airway inflammation. Subsequently we observed the mice for signs of inflammation and investigated Th1-, Th2-, and Treg-related cytokine levels as well as recruitment of Treg cells into the airway.ResultsWe found that ASC sup could ameliorate allergic airway inflammation in this model; the value of airway hyperresponsiveness, and the occurrence of inflammatory cell infiltration in the lung, as well as the number of eosinophils, and goblet cells in the lung epithelium were all significantly decreased by ASC sup treatment. In addition, ASC sup treatment significantly decreased the levels of IL-4, IL-5, and IL-13 in the bronchial alveolar lavage fluid and in culture medium of lung-draining lymph node cells of the animal model of acute asthma. We detected numerous CTLA-4 and Foxp3-expressing cells in the lung after ASC sup treatment. ASC sup was found to have a higher concentration of IL-10 and TGF-β compared to con sup.ConclusionsStem cells have powerful potential for therapeutic functions in various diseases, but they also have many drawbacks. In this study, we found strong immunosuppressive ability of ASC sup in an allergic airway mouse model. It may be possible to use ASC sup for treatment of many immunological diseases in the near future.
Highlights
In a previous study, we demonstrated that intravenous administration of adipose tissue stem cells (ASCs) could significantly reduce allergic symptoms and suppress eosinophilic inflammation
We demonstrated that intravenous administration of ASCs could reduce allergic symptoms significantly and suppress eosinophilic inflammation [6, 7]
Treatment with ASC sup suppressed OVA-induced airway inflammation To demonstrate the effects of ASC sup on OVA-induced airway inflammation, we pretreated the airways of an OVA-induced mouse model with ASC sup, and assessed biological and pathological changes (Fig. 1a)
Summary
We demonstrated that intravenous administration of adipose tissue stem cells (ASCs) could significantly reduce allergic symptoms and suppress eosinophilic inflammation. The expansive abilities of MSCs make them one of the most important candidates for disease therapy and regenerative medicine. They have strong immunosuppressive activity in different situations, and clinical trials are ongoing evaluating them for treatment of immunological disease [2,3,4]. We demonstrated that intravenous administration of ASCs could reduce allergic symptoms significantly and suppress eosinophilic inflammation [6, 7]. CD4+CD25+Foxp3+ T cells (regulatory T cells, Yu et al Stem Cell Research & Therapy (2017) 8:8
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
