Abstract
Large quantities of Rhizobium leguminosarum by conventional technique are a time-consuming and labor-intensive method of production. The selection of different types of nutrient media for the production of large amounts of rhizobium at low cost, determination of environmental regulators of culture medium and the effect of different levels of air flow into nutrition media have been investigated. Rhizobium cultured in modified air lift bioreactor as improved conventional system using Yeast Extract Mannitol Broth by assessing aeration and agitation in their submerged growth. For selection of suitable nutrient media for Rhizobium before mass production, YMA (Yeast Mannitol Agar), YSA (Yeast Sucrose Agar), YMA + bromthymol blue (BTB), YMA + Congo red (CR) medium was tested. To optimize the growing conditions of different temperatures, pH levels and salt strengths were investigated. Three air flow speeds at the rate of 0.01, 0.05 and 0.1 VVM for oxygen supply were ensured. A thick off-white bacterial colony was observed on the YMA plate among different culture media. Room temperature (25˚C - 30˚C), neutral pH (7.0), low salinity (1%) was observed to be suitable among the environmental conditions for Rhizobium mass production. The maximum density of Rhizobium was observed using air supply of 0.1 VVM level in bioreactor system. The result seemed that the air supply and agitation was an important growth factor in submerged cultivation. This study provides useful information on large scale production of Rhizobium of interest employing low cost effective modified air lift bioreactor within less time.
Highlights
In terms of business the term, “large scale production” refers to the production of a commodity on a large scale with a large sized firm
This study provides useful information on large scale production of Rhizobium of interest employing low cost effective modified air lift bioreactor within less time
In this research bioreactor for Rhizobium large scale production is usually described in a biochemical sterile environment that capitalizes on liquid nutrient or liquid/air flow and outflow systems, designed for intensive culture and control over micro environmental conditions
Summary
In terms of business the term, “large scale production” refers to the production of a commodity on a large scale with a large sized firm. High-capacity shakers capable of handling numerous flasks at one time are available, they are expensive This necessitates the use of smaller, cheaper orbital shakers that requires multiple rounds of culturing to generate enough material for compound isolation and characterization [3]. Conventional funnel or balloon shaped glass containers are taken for the purpose of cultivating more bacteria in a short period of time It is not cost effective as low cost parts are assembled. Aimed at mass production in full in vitro condition to optimization of culture media, culture conditions including agitation for liquid form of Rhizobium, multiply the Rhizobium largely in modified air-lift photo bioreactor with less time and cost
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