Abstract
Summary We investigated the conditions required for successful culture of sugar beet ( Beta vulgaris L.) protoplasts. Mesophyll-derived protoplasts regenerated cell walls and underwent division when embedded in alginate (Sigma, low viscosity, 1 %) and cultivated on a modified Schenk and Hildebrandt (SH) medium (1.5 mg/L 2,4-dichlorophenoxyacetic acid, 0.3 mg/L 6-benzylaminopurine, 0.1 mg/L 1-naphthaleneacetic acid, 0.4 M glucose) in the presence of nurse cells (sugar beet suspension cells). The plating efficiency increased up to 3.7 % when the protoplasts were given a cold pretreatment in a salt solution (8 °C, 20 min) prior to embedding in alginate. Colonies derived from mesophyll protoplasts frequently showed root organogenesis on a medium containing 1-naphthaleneacetic acid, 6-benzylaminopurine or tri-iodobenzoic acid, respectively, or on hormone-free medium.
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