Abstract
The study was carried out to establish an EV71 virus culture system on Vero cells in a shake culture system (Wave bioreactor) incorporating microcarriers for the production of hand, foot and mouth (HFM) vaccine caused by the EV71 virus. The results of the investigation of 2 types of microcarriers (Cytodex 1 and Cytodex 3), showed that Cytodex 1 gave better efficiency when culturing Vero cells in suspension conditions. The combination with Cytodex 1 on the Wave bioreactor system gave Vero cell culture efficiency of over 3x106cells/ml. The optimal infectious dose of EV71 virus on Vero cells was determined to be 0.0001 TCID50/ml. The optimisation of EV71 virus culture conditions on the Wave bioreactor system has obtained suitable results for the production of vaccine with virus titres 108TCID50/ml on the fifth day after infection. The procedure of production of EV71 virus on Vero cell in Wave bioreactor system was applicable in vaccine production with virus titres 108 TCID50/ml on the fifth day after infection.
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