Culture of EV71 virus in wave bioreactor system for production of hand, foot and mouth disease vaccine

Abstract

The study was carried out to establish an EV71 virus culture system on Vero cells in a shake culture system (Wave bioreactor) incorporating microcarriers for the production of hand, foot and mouth (HFM) vaccine caused by the EV71 virus. The results of the investigation of 2 types of microcarriers (Cytodex 1 and Cytodex 3), showed that Cytodex 1 gave better efficiency when culturing Vero cells in suspension conditions. The combination with Cytodex 1 on the Wave bioreactor system gave Vero cell culture efficiency of over 3x106cells/ml. The optimal infectious dose of EV71 virus on Vero cells was determined to be 0.0001 TCID50/ml. The optimisation of EV71 virus culture conditions on the Wave bioreactor system has obtained suitable results for the production of vaccine with virus titres 108TCID50/ml on the fifth day after infection. The procedure of production of EV71 virus on Vero cell in Wave bioreactor system was applicable in vaccine production with virus titres 108 TCID50/ml on the fifth day after infection.