Culture of Continuous Cell Lines

Abstract

AbstractThis chapter describes the culture of various continuous cell lines. Continuous cell lines are generated either due to specific genetic or epigenetic alterations in the genome of certain cells. The reasons for genetic alterations are the manifestation of spontaneous or induced mutations via radiations and chemical or biological agents such as viruses. In the experimental laboratory conditions, continuous cell lines can be generated by a viral infection, biochemical agents, or radiations. This chapter discusses the culture of major mammalian continuous/cancer cell lines, including breast cancer cell line (MCF-7 and MDA-MB-231 cell), ovarian cancer cell line (CHO cells), prostate cancer cell line (LNCaP and PC3 cells), cancer cell lines originating from the female cervix (HeLa cells), lung cancer cell lines (A549 and BEAS-2B cells), kidney cancer cell lines (HEK-293 and VERO cells), liver cancer cell lines (HepG2 cells), and blood cancer cell lines (JURKAT and RAW cells). While some of the abovementioned cancer cell lines are isolated directly from various cancer patients, others are experimentally generated in the laboratory. The knowledge gained from this chapter would help to understand the culture and regular maintenance of the abovementioned continuous cell lines and in the development of various anticancer agents.KeywordsCulture of Continuous /Cancer Cell LinesMCF-7 Breast Cancer Cell LineMDA-MB-231 Breast Cancer Cell LineCHO Ovarian Cancer Cell LineLNCaP Prostate Cancer Cell LinePC3 Prostate Cancer Cell LineHeLa Cervix Cancer Cell LineA549 Lung Cancer Cell LineBEAS-2B Lung Cancer Cell LinesHEK-293 Kidney Cancer Cell LinesVERO Kidney Cancer Cell LineHepG2 Liver Cancer Cell LineJURKAT Blood Cancer Cell LinesRAW Blood Cancer Cell Lines