Culture media and bap concentrations in the embryo culture of ‘BRS Kampai’ peach

Chaiane Renata Grigolo,Idemir Citadin,Marisa De Cacia Oliveira,Ester Provensi Santos,Silvia Scariotto,Rafael Henrique Pertille

doi:10.1590/0100-29452020661

Chaiane Renata Grigolo, Idemir Citadin + Show 4 more

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https://doi.org/10.1590/0100-29452020661

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Abstract

Abstract Early-ripening peach cultivars present difficulties in the process of natural germination of seeds. In order to solve this problem, the culture of embryos in vitro can be considered an alternative, in which seeds find conditions to complete germination and development in a satisfactory way. Different protocols and culture media have been tested to meet the nutritional needs of the embryo, but they still have problems and need to be optimized. The objective of this work was to test culture media and BAP concentrations in order to increase the germination percentage of embryos and viable ‘BRS Kampai’ peach seedlings, avoiding possible anomalies in the process. The experiment was carried out at the Laboratory of Tissue Culture – “Universidade Tecnológica Federal do Paraná”, Campus of Pato Branco. The experimental design used was randomized blocks, in 2x5 factorial, with four replicates, with plot being represented by 15 embryos. The culture media tested were MS and WPM with five BAP concentrations (0, 1, 2, 3 and 4 mg L-1). In the in vitro period, seed germination was evaluated through the attribution of scores, and embryo development, measuring the length of stem and main root. During the acclimatization period, seedlings were evaluated for survival, stem length, viable seedlings and rosette formation. Considering the conditions under which the experiment was conducted, it was concluded that MS culture medium with the addition of 1 mg L-1 of BAP allowed higher germination percentage and viable ‘BRS Kampai’ peach seedlings.

Highlights

IntroductionConsiderando as condições sob as quais o experimento foi conduzido, concluiu-se que o meio de cultura MS com a adição de 1 mg L-1 de BAP permitiu uma maior porcentagem de germinação e plântulas viáveis para ‘BRS Kampai’
‘BRS Kampai’ peach tree (RASEIRA et al, 2010) is a cultivar launched by “Embrapa Clima Temperado” stone fruit breeding program in Pelotas, which has been widely cultivated and has shown great adaptability and productive stability (SCARIOTTO et al, 2013; CITADIN et al, 2014). It appears that seeds of earlyripening peach cultivars, such as ‘BRS Kampai’, do not germinate due to the immaturity condition of the embryo (SUNDOURI, 2014) and to the accelerated growth and ripening of fruits that does not allow time for the embryo to reach the ideal size and physiological maturation (RAMMING et al, 2003)
Several nutritional media have been used in the in vitro rescue of Prunus embryos, most of which based on MS medium and its variations (GERCHEVA et al, 2002; LIU et al, 2007; REIS et al, 2012; SUNDOURI et al, 2014; MANSVELT et al, 2015; DEVI et al, 2017) and WPM (RAMMING et al, 2003; RASEIRA; EINHARDT, 2010; NASCIMENTO et al, 2017)

Summary

Introduction

Considerando as condições sob as quais o experimento foi conduzido, concluiu-se que o meio de cultura MS com a adição de 1 mg L-1 de BAP permitiu uma maior porcentagem de germinação e plântulas viáveis para ‘BRS Kampai’. ‘BRS Kampai’ peach tree (RASEIRA et al, 2010) is a cultivar launched by “Embrapa Clima Temperado” stone fruit breeding program in Pelotas, which has been widely cultivated and has shown great adaptability and productive stability (SCARIOTTO et al, 2013; CITADIN et al, 2014) It appears that seeds of earlyripening peach cultivars, such as ‘BRS Kampai’, do not germinate due to the immaturity condition of the embryo (SUNDOURI, 2014) and to the accelerated growth and ripening of fruits that does not allow time for the embryo to reach the ideal size and physiological maturation (RAMMING et al, 2003). The cytokinin most used for this purpose is BAP (6-benzylaminopurine) (VILLA et al, 2006) due to its efficiency in the multiplication of shoots (HU; WANG, 1983)

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