Abstract
Amplification protocol in vitro was developed for multiplication of Valeriana officinalis L. var. latifolia Miq. by using leaves, petiols, rhizome and roots derived from wild plants grown in pot as explants. Effects of different plant growth regulators on callus induction, adventitious bud differentiation and bud anatomy were studied. The results showed that it was optimal for the explants to be disinfected with 70% ethanol for 30 s and then soaked with 0.1% mercuric chloride for 6 min, the optimum medium for callus induction was MS+2,4-D 2 mg/L +6-BA 0.5 mg/L, and callus induction rate of leaves was up to 80%. However, buds were hard to regenerate from callus, and the highest differential rate was 25% on the medium of MS+6-BA 4 mg/L+NAA 0.5 mg/L. Buds regenerated exogenous from callus epidermal cells.
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