Abstract

Qualitative characteristics of changes in culture fluorescence during batch growth ofClostridium acetobutylicum [ATCC 824] have been determined. A solventogenic phase culture shows higher changes in normalized fluorescence levels per unit change in cell concentration compared with an acidogenic phase culture. This suggests that during solvent formation intracellular NADH levels are higher than in the acidogenic phase and that NADH availability may influence the onset of solventogenesis.

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