Abstract
AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials AME 81:171-188 (2018) - DOI: https://doi.org/10.3354/ame01863 Culture-dependent and -independent methods reveal dominance of halophilic Euryarchaeota in high-altitude Andean lakes Marcos J. Maldonado1,2, Virginia H. Albarracín1,3, José A. Lara1, Marcela A. Ferrero1,4, María E. Farías1,* 1Laboratorio de Investigaciones Microbiológicas de Lagunas Andinas (LIMLA), PROIMI Planta Piloto de Procesos Industriales Microbiológicos, Av. Belgrano y Pasaje Caseros, 4000 CONICET Consejo Nacional de Investigaciones Científicas y Técnicas, 4000 Tucumán, Argentina 2Facultad de Ciencias Agrarias, Alberdi 47, Universidad Nacional de Jujuy, 4600 Jujuy, Argentina 3Facultad de Ciencias Naturales e Instituto Miguel Lillo, Miguel Lillo 205, Universidad Nacional de Tucumán, 4000 Tucumán, Argentina 4Facultad de Bioquímica, Química y Farmacia, Ayacucho 471, Universidad Nacional de Tucumán, 4000 Tucumán, Argentina *Corresponding author: mefarias@proimi.org.ar ABSTRACT: The hypersaline and extreme environments of the so-called high-altitude Andean lakes in the dry central region of the Andes are considered pristine ecosystems of biotechnological interest. By using culture-dependent and -independent methods, we aimed to describe the phylogenetic affiliation and ecological importance of Archaea thriving in extreme, hypersaline lakes and salt flats in the Argentinean and Chilean Puna. For this purpose, water and sediment samples were collected from 14 lakes and salt flats. Denaturing gradient gel electrophoresis (DGGE) indicated that representatives of the phylum Euryarchaeota dominated the microbial community in all samples. Most of the DGGE bands were assigned to the family Halobacteriaceae, while the rest corresponded to Methanocaldococcaceae, Methanobacteriaceae, Methanococcaceae, Methanosarcinaceae, and Methanothermaceae. In addition, isolation procedures yielded single colonies of 53 pure isolates belonging to the following genera: Haloarcula, Halomicrobium, Halopiger, Halorubrum, Natrialba, Natrinema, Natronorubrum, and Natronococcus. All strains proved to be polyextremophiles, with high tolerance to NaCl, UV-B radiation, and high arsenic concentration. KEY WORDS: Archaea · Extremophiles · Halophiles · Arsenicv · UV · Andean Lakes · Puna Full text in pdf format Supplementary material PreviousNextCite this article as: Maldonado MJ, Albarracín VH, Lara JA, Ferrero MA, Farías ME (2018) Culture-dependent and -independent methods reveal dominance of halophilic Euryarchaeota in high-altitude Andean lakes. Aquat Microb Ecol 81:171-188. https://doi.org/10.3354/ame01863 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in AME Vol. 81, No. 2. Online publication date: April 17, 2018 Print ISSN: 0948-3055; Online ISSN: 1616-1564 Copyright © 2018 Inter-Research.
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