Culture dependent analysis of bacterial activity, biofilm-formation and oxidative stress of seawater with the contamination of microplastics under climate change consideration

Abstract

Temperature changes due to climate change and microplastic contamination are worldwide concerns, creating various problems in the marine environment. Therefore, this study was carried out to discover the impact of different temperatures of seawater exposed to different types of plastic materials on culture dependent bacterial responses and oxidative characteristics. Seawater was exposed to microplastics obtained from various plastic materials at different temperature (−18, +4, +20, and +35 °C) for seven days. Then microplastics were removed from the suspension and microplastic-exposed seawater samples were analyzed for bacterial activity, biofilm formation and oxidative characteristics (antioxidant, catalase, glutathione, and superoxide dismutase) using Gram-negative Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus. The results showed that the activity and biofilm formation of Pseudomonas aeruginosa and Staphylococcus aureus were affected through oxidative stress by catalase, glutathione, and superoxide dismutase due to the microplastic deformation by temperature changes. This study confirms that temperature changes as a result of climate change might influence microplastic degradation and their contamination impact in seawater in terms of bacterial metabolic and oxidation reactions.