Abstract
Summary Mesophyll protoplasts were isolated using a Biogel-purified enzyme mixture from leaflets of vegetative and flowering stems of Trigonella corniculata and T. foenum-graecum grown in a controlled environment cabinet. Protoplasts of both species divided in liquid medium with a plating efficiency of 70%. Cell suspensions initiated from leaf callus of T. corniculata yielded protoplasts which formed callus. In contrast, protoplasts were not obtained from cell suspensions of T. foenum-graecum . Callus derived from leaf explants, mesophyll protoplasts and cell suspension protoplasts of T. corniculata formed embryoids and shoots, but tissues of T. foenum-graecum were recalcitrant to regeneration. These results, including the difficulties associated with T. foenum-graecum , are discussed in relation to recent progress on regeneration in forage legumes.
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