Abstract
In connection with a serologic study 1 of the gonococcus it was necessary for us to devise methods and mediums whereby this organism might be isolated readily from gonorrheal infections, and also a large collection of strains might be maintained with a minimal risk of any of them being lost. The difficulties inherent in the cultivation of the gonococcus are evidenced by the great variety of mediums and procedures which have been recommended for this purpose, especially within recent years. At present there seems to be no consensus of opinion as regards the best methods to use. In the following section certain simple mediums are described which have given good results consistently in connection with the various purposes specified. It may be remarked that through the use of a sterilizable medium of proper reaction hardly greater difficulty has been encountered in maintaining a large collection of gonococcus strains than would be associated with carrying a like number of typhoid strains.
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