Cultivation of Viral Diarrhea Virus in Continuous Cell Cultures and Assessment of Its Infectious Activity

Abstract

AbstractPestiviruses are enveloped viruses of the Flaviviridae family, which have a highly variable genome, which is represented by a single-stranded (+) RNA with a size of ≈12.3 thousand of nucleotide sequences (1). The genus Pestivirus includes the established species Bovine Diarrhea Virus (BVDV) –1, BVDV-2, Borderline Disease Virus (BDV) and Classical Swine Fever Virus (CSFV), as well as a growing number of additional suspected Pestivirus species. Bovine viral diarrhea virus is widespread in many countries of the world. The economic damage is significant: the shortage of offspring due to abortion, stillbirth, treatment costs, identification of persistently infected animals and prevention, especially among young livestock. One of the methods of disease diagnostics is the method of virus isolation in cell culture. The paper presents the results of the study of biological properties of the reference viral diarrhea virus Oregon 24 and Ressa isolate of cattle identified as the first type. Viruses were cultured in continuous cell cultures PT-80, LEK, KST, Taurus-1 (T-1) for 5 passages. Virus titers were counted in every 5th passage, which ranged from 5.5–5.75 lg TCID50/0.1 ml for PT-80, KST, Taurus-1 cell cultures, for the continuous LEK line from 3.00 to 4.75 lg TCID50/0.1 ml.KeywordsContinuous cell cultures PT-80LEKKSTTaurus-1 (T-1)Viral diarrhea virusOregon 24 strainRessa viral diarrhea virus isolate