Cultivation of two Chlorella species in Open sewage contaminated channel wastewater for biomass and biochemical profiles: Comparative lab-scale approach

Abstract

Open sewage contaminated channel wastewater (OSCCW) has high pollutant loads, responsible for eutrophication, when mixed with various channels of urban communities. But, these pollutants can be converted and recovered into useful end products with the help of algal species.In this study, two species of Chlorella (C. vulgaris and C. pyrenoidosa) were selected and investigated for the production of algal biomass and nutrient removal efficiencies with 50% concentration of OSCCW, in a comparative way at lab-scale. Chlorella sp. cultivated in OSCCW have removed nitrate (76.9–78.8%) and phosphate (67.6–79.7%) whereas COD (72.4–76.2%) and BOD (62.3–72.4%) respectively. Correlation analysis was investigated between physico-chemical parameters and biochemical profile of both species to analyze the positive and negative correlation between two variables. The bio-chemical profile and biomass productivity of both species of Chlorella were observed well on the basis of productivity of biomass (60.1, 56.5 mg/l/d), carbohydrate (15.71, 8.82 mg/l/d), protein (11.21, 15.82 mg/l/d), lipid (20.8, 17.5 mg/l/d) and chlorophyll (0.78, 0.67 mg/l/d) in OSCCW. The maximum lipid content (34.6%) was obtained with C. pyrenoidosa as compared to C. vulgaris. Findings also support that OSCCW is well-off with nutrient resources, which can be suitable alternative for algal biomass production and remediated wastewater can be used for animal and fish farming type activities.