Cultivation of transgenic rice plants with OsCDPK7 gene and its salt tolerance

Abstract

A 1,700 bp DNA fragment, OsCDPK7 gene, was cloned with RT-PCR from liaoyan241 leaf treated under a low temperature of 4. Compared to the OsCDPK7 gene reported before (GenBank accession No. AB042550), this fragment, lack of 26 amino acids, possesses the activity of Ca2+-dependent protein kinase because of a complete integration of the Ca2+ binding structure domain and Ser/Thr protein kinase activity center. Plant expression vector was constructed,, OsCDPK7 gene was regulated by E12 promoter. OsCDPK7 gene was transferred into rice via Agrobacterium-mediated method. After Km screening and Southern blot, 10 transgenic plants were obtained. The analysis on the salt tolerance showed that the expression of OsCDPK7 gene composition enhanced the salt tolerance of T2 transgenic plants, part of T2 transgenic seeds could germinate in 0.2 mol/L NaCl medium, and T2 transgenic young plants could rejuvenate after treatment with 0.4 mol/L NaCl for 10 days, while the controlled plants could not germinate and died in salt stress. This research finding proved that the regulation factor of the plant signal transduction could enhance the salt tolerance of transgenic plants, while OsCDPK7 expression was different in the different tolerence transgenic plants.