Abstract
Glucose belongs among the most important substances in both physiology and industry. Current food and biotechnology praxis emphasizes its on-line continuous monitoring and regulation. These provoke increasing demand for systems, which enable fast detection and regulation of deviations from desired glucose concentration. We demonstrated control of glucose concentration by feedback regulation equipped with in situ optical fiber glucose sensor. The sensitive layer of the sensor comprises oxygen-dependent ruthenium complex and preimmobilized glucose oxidase both entrapped in organic–inorganic polymer ORMOCER®. The sensor was placed in the laboratory bioreactor (volume 5 L) to demonstrate both regulations: the control of low levels of glucose concentrations (0.4 and 0.1 mM) and maintenance of the glucose concentration (between 2 and 3.5 mM) during stationary phase of cultivation of Saccharomyces cerevisiae. Response times did not exceed 6 min (average 4 min) with average deviation of 4%. Due to these regulation characteristics together with durable and long-lasting (≥2 month) sensitive layer, this feedback regulation system might find applications in various biotechnological processes such as production of low glucose content beverages.
Highlights
Accepted: 11 January 2021The number of people with diabetes has risen from 108 million in 1980 to 422 million in 2014, and between 2000 and 2016 there was a 5% increase in premature mortality from diabetes the World Health Organization (WHO) predicts that the diabetes will be the seventh leading cause of death in 2030 [1]
In case the desired glucose concentration was equal to limit of de7 of 15 tection (LOD) of used biosensor, the average deviation increased to 3.7%
In case the desired glucose concentration was equal to limit of detection (LOD) of used biosensor, the average deviation increased to 3.7%
Summary
Accepted: 11 January 2021The number of people with diabetes has risen from 108 million in 1980 to 422 million in 2014, and between 2000 and 2016 there was a 5% increase in premature mortality from diabetes the World Health Organization (WHO) predicts that the diabetes will be the seventh leading cause of death in 2030 [1]. The accurate evaluation of the glucose content in foods is extremely important for the maintenance of its physiological level in blood of diabetic individuals. Microwave resonatorbased sensors might be advantageous for glucose detection in blood [5,6] or in some industrial applications as their linear range of measured glucose concentrations is from zero to more than ten weight percent [7]. In comparison with these microwave-based sensors, optical biosensors with glucose oxidase exhibit a high specificity to glucose
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