Cultivation of Medicinal Mushrooms Ganoderma lucidum and Grifola frondosa Mycelia and Polysaccharides in Bioreactors

Abstract

Original strains of Ganoderma lucidum ( MZKI G97) and Grifola frondosa (GF3) isolated from Slovenian forests were cultivated using submerged and solid state cultivation. In 14 days submerged Ganoderma lucidum fed batch cultivation extracellular (1,7 g/L) and intracellular (0.45 g/L) polysaccharide fractions were isolated, up to 17.0 g/L dry fungal biomass was produced, while in 28 days Grifola frondosa fed batch cultivation 3.65 g/L of extracellular and 1.30 g/L intracellular polysaccharide and 15.2 g/L dry biomass was produced. In Ganoderma lucidum solid state cultivation in 18 days 5.77 mg /g extracellular and 1.45 mg /g intracellular polysaccharide was produced while in Grifola frondosa in 38 days cultivation of 0.70 extracellular and 3.80 mg/g of intracellular polysaccharide were produced. The isolated polysaccharides were mainly -D-glucanes. Immunostimulatory effects of isolates were tested on induction of cytokine (TNF- , IFN- and IL12) synthesis in primary cultures of human mononuclear cells (PBMC) isolated from a buffy coat.