Abstract
A simple method1, 2 for the cultivation of vaccine virus was described which consisted of minced viable chick embryo tissue suspended either in Tyrode's solution or in a mixture of Tyrode's solution and rabbit serum. Recently it has been shown that the viruses of vesicular stomatitis3 and poliomyelitis4 are capable of multiplication in media similar to that used for vaccine virus. For the cultivation of the former active agent, minced chick embryo tissue suspended in Tyrode's solution was used, while for the latter minced chick embryo brain tissue suspended in a mixture of monkey serum (1 part) and Tyrode's solution (9 parts) was employed. During the past year we have been investigating louping ill, a neurotropic virus disease of sheep which in some respects resembles poliomyelitis. After the appearance of Gildemeister's note4 on the cultivation of poliomyelitic virus, it seemed of interest to determine whether another neurotropic virus, the etiological agent of louping ill, is capable of growth in vitro ...
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