Abstract
SummaryThe imaginal discs of fourth instar mosquito larvae (Aedes aegypti) and the male and female gonads of diapausing Cynthia pupae were cultivated in vitro. Mosquito larval tissues cultivated in a basic medium, designed for insect tissue culture, yielded a very low percentage of cultures with primary outgrowth. The incorporation into this medium of 10% chick embryo extract increased considerably the number of positive cultures. The incorporation into the medium of extracts from homologous insects had no beneficial effect on growth of tissues from either insect. The cells from the imaginal discs of mosquito larvae supported the proliferation of West Nile virus, whereas the cells from the Cynthia gonads did not.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: The American journal of tropical medicine and hygiene
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
