Abstract
The genus Entamoeba contains many species, three of which are identical (Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii ) reside in the human intestinal lumen. Entamoeba histolytica is the causative agent of amebiasis and mortality worldwide in humans. Although recent studies highlight the recovery of E. dispar andE. moshkovskii from patients with gastrointestinal symptoms. The cultivation of three identical Entamoeba species was done successfully in Lock’s-egg medium , a high number of trophozoite were found in culture .these three identical Entamoeba species did not differentiation by using microscope .but the devolution of scientific they could differentiation by using PCR technique when extraction the DNA in stool samples by using specific bands and primers
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