Abstract
Cell cultures derived from bovine mammary glands have been cultivated in the laboratory for varying periods including some which have been propagated for up to two and a half years and have had 30 to 40 passages. For many months most of the cultures are composed of stable populations of predominantly epithelial-like cells which have a generation time of about four days. These cultures retain considerable organizational ability with the fibroblastic-like cells. As the cultures age, most tend to become more fibroblastic-like and to lose their organizational ability. Glycogen synthesis has been observed to be more active in the younger and more epithelial-like cultures. Even though the cells do not have a rapid generation time, the period of stabilized growth is long enough so that almost unlimited numbers of cells can be obtained for use in controlled cell studies.
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