Cultivar differences in the operation of the photosystem II of barley leaves

Eugene Lisitsyn,Irina Shchennikova,Svetlana Churakova,N.A Shyurova,O.V Tkachenko

doi:10.1051/bioconf/20224301001

Abstract

Differences between nine spring barley cultivars on structural and functional parameters of PSII operation are shown. Variability of the parameters used changed from 2.2…2.5 (oxidized quinones pool and maximum photochemical efficiency) to 24.6% (performance index PIABS_total). Parameters characterizing the values of excitation energy fluxes (ABS/RC; TRo/RC; ETo/RC) varied to a small extent - 3.1...6.5%, while the lost energy (DIo/RC) much stronger - 14.6%. The efficiency of electron transport from quinine QB and the entire PSII until the PSI’s primary acceptors varied even more - 18.1 and 17.4%, respectively. The PIABS integral performance index combined cultivars into two groups only; PIABS_total index showed more clear differences in cultivars. Cv. Novichok and Fermer were significantly inferior to cv. Forward, Belgorodsky 100, Boyarin and Bionic in most of the studied parameters. However, according to the results of a cluster analysis, cv. Novichok and Forward turned out to be similar to each other, as well as cv. Fermer, Boyarin and Bionic, which separated into the same cluster. Probably, the action of PSII in cultivars of the same cluster differs only quantitative but not qualitative. Therefore, for breeding work on pyramidizing the genes controlling operation of this photosystem, it is advisable to crossing cultivars belonging to different clusters.

Highlights

The level of photosynthetic reactions is a good measure of the total plant productivity
Chlorophyll a (Chl a) fluorescence is a measure of the energy of absorbed light quants that have not been used in photosynthesis or dissipated as heat [1]
The study showed the existence of statistically significant differences between spring barley cultivars on structural and functional parameters of the action of photosystem II (PSII) in leaves of two-week-old seedlings

Summary

Introduction

The level of photosynthetic reactions is a good measure of the total plant productivity. The fastest and most economical method of measuring the photosynthetic processes of plants is the method of assessing chlorophyll fluorescence. In normal and efficient operation of the photosystem 2 (PSII), the intensity of chlorophyll a fluorescence remains low, and any disruption of the photosynthesis process leads to its significant increase [2]. The change in the parameters of rapid fluorescence of barley leaves under the influence of drought has been studied in [4, 5]; under the influence of high temperature - in [6], under the influence of salinity - in [7]. On the example of two barley cultivars, [10] estimated the influence of different intensity of light, high and low temperature, cadmium, lead, chloride salinity, deficiency of macro- and microelements of nutrition on the activity of PSII. Some articles are published showing the effectiveness of this technique in assessing the level of resistance against fusariosis in some agricultural species including barley [11]

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Discussion

Conclusion