Cultivar differences in soluble sugar content of mature rice grain

Abstract

Mature grain from 31 rice (Oryza sativa L.) cultivars grown in the same location was analyzed for soluble sugar content to determine quantitative differences in this parameter. Cultivar variation in caryopsis sucrose content was 4‐fold ranging from 15 to 59 μmol sucrose (g fresh weight)−1. Soluble reducing sugar ranged from 7 to 15 μmol hexose (g fresh weight)−1. Soluble sugar content was much greater in the outer part of the grain than in the endosperm. Caryopsis enzyme activities were measured in 8 cultivars having a range of grain sucrose content. No relationship between grain sucrose content of these cultivars and the level of enzymes of sucrose metabolism, glycolysis, and oxidative pentose pathway was established. Caryopsis respiration after 1 h of imbibition also was not related to the amount of soluble sugars available in the grain among these 8 cultivars. The results show that there are significant differences in the sugar‐accumulating capacity of the caryopsis of different rice cultivars. The detection of a fructose 2,6‐bisphosphate‐sensitive, PPi: fructose 6‐phosphate phosphototransferase (EC 2.7.1.90) in the endosperm suggests the presence of a regulatory mechanism for sucrose/starch partitioning established in other plant tissues.