Abstract

Seed purity is a key indicator of crop seed quality. The conventional methods for cultivar identification are time-consuming, expensive, and destructive. Fourier transform near-infrared (FT-NIR) spectroscopy combined with discriminant analyses, was studied as a rapid and nondestructive technique to classify the cultivars of sweet corn seeds. Spectra with a range of 1000–2500 nm collected from 760 seeds of two cultivars were used for the discriminant analyses. Thereafter, 126 feature wavelengths were identified from 1557 wavelengths using a genetic algorithm (GA) to build simplified classification models. Four classification algorithms, namely K-nearest neighbor (KNN), soft independent method of class analogy (SIMCA), partial least-squares discriminant analysis (PLS-DA), and support vector machine discriminant analysis (SVM-DA) were tested on full-range wavelengths and feature wavelengths, respectively. With the full-range wavelengths, all four algorithms achieved a high classification accuracy range from 97.56% to 99.59%, and the SVM-DA worked better than other models. From the feature wavelengths, no significant decline in accuracies was observed in most of the models and a high accuracy of 99.19% was still obtained by the PLS-DA model. This study demonstrated that using the FT-NIR technique with discriminant analyses could be a feasible way to classify sweet corn seed cultivars and the proper classification model could be embedded in seed sorting machinery to select high-purity seeds.

Highlights

  • The sweetness of sweet corn is a major factor in consumer satisfaction, which attracts a high interest from breeders to breed sweeter corn cultivars [1]

  • For the cultivar Huameitian No.8 (H8), its female parent was bred from the sweet corn cultivar 03AX-538BC, which originated from the USA

  • For the cultivar Huameitian No.168 (H168), its female parent was bred from another sweet corn cultivar ACX232, which originated from the USA

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Summary

Introduction

The sweetness of sweet corn is a major factor in consumer satisfaction, which attracts a high interest from breeders to breed sweeter corn cultivars [1]. Some characteristic genes of sweet corn cultivars, such as sugary-1, sugary enhanced, and shrunken, can make the conversion of sugar to starch negligible before the kernels fully ripen [3]. For this reason, a uniform maturity time is crucial for farmers to choose the optimal harvest time, and to obtain a proper shelf-life quality as the sweet flavor of sweet corn changes quickly after harvesting [4]. After removing other cultivars of sweet corn seeds, the tidy harvest time can be obtained with the pure cultivar seeds. Because the economic value, nutritional value, and resistance to diseases and pests of sweet corn are related to

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