Abstract
The inclusion complex formation of nereistoxin (NTX) with cucurbit[7] uril (CB[7]) in aqueous solution was investigated by competitive binding of palmatine (PAL) fluorescent probe. The CB[7]–PAL complex exhibited high fluorescence in aqueous solution, which was quenched in the presence of NTX. This is due to the extrusion of PAL from the CB[7] cavity into the aqueous phase by NTX. The association constants ( K) for the inclusion complexation of the host and guest were determined. The encapsulation of NTX and PAL as they compete for occupancy of the CB[7] cavity was studied using absorption, spectrofluorimetry, 1H NMR, and molecular modelling calculations.
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