Abstract
SUMMARY Protoplasts isolated from Nicotiana tabacum (cv. Xanthi) leaves were infected with cucumber green mottle mosaic virus (CGMMV). Progeny CGMMV particles were first detected in the protoplasts at 24 h after inoculation. They were in small crystalline arrays confined in the ground cytoplasm. The earliest detectable cytological modification in the protoplasts was the presence of peripheral vesicles in the mitochondria at 24 h after inoculation. No other effects on the cytoplasm or organelles were noted.
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