CUB and Sushi Multiple Domains (CSMD1) Gene Polymorphisms and Susceptibilities to Idiopathic Parkinson's Disease in Northern Chinese Han Population: A Case-Control Study.

Xinling Bai,Jianing Jin,Anmu Xie,Shanshan Li,Huimin Wang,Pablo Mir

doi:10.1155/2021/6661162

Xinling Bai, Jianing Jin + Show 4 more

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https://doi.org/10.1155/2021/6661162

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Abstract

Evidence has shown that the CUB and Sushi Multiple Domains (CSMD1) gene is an inhibitor of the complement activation pathway and is also involved in central nervous system inflammation. Previous studies have revealed that the CSMD1 gene is related to familial Parkinson's disease. This study aimed to investigate the relationship between CSMD1 gene and susceptibility to Parkinson's disease in population of northern China. A case-control study was performed on 423 Parkinson's disease patients and 465 healthy controls matched for age and sex. DNA from enrolled subjects were extracted from the peripheral blood, and single nucleotide polymorphisms (SNPs) rs12681349 (C＞T), rs10503253 (C＞A), and rs1983474 (T＞G) within CSMD1 gene were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Genotype frequency of rs10503253 (CA versus CC : OR = 1.554, 95% CI = 1.169–2.066, p=0.002) and rs1983474 (GG versus TT : OR = 0.599, 95% CI = 0.401–0.895, p=0.012) was significantly different between PD cases and controls, but not for rs12681349. Comprehensive and subgroup analysis indicated that rs10503252 showed significant statistical differences in the dominant model (AA + CA versus CC : OR = 0.677, 95% CI = 0.517–0.886, p=0.004), late-onset cohort (CA versus CC : OR = 1.570, 95% CI = 1.159–2.126, p=0.004), and the female cohort (CA versus CC : OR = 0.687, 95% CI = 0.497–0.952, p=0.023), compared with the matched control group. The difference of recessive model of rs1983474 (GG versus TT + TG : OR = 1.837, 95% CI = 1.287–2.620, p=0.001) was significant in Parkinson's disease. According to the subgroup analysis, results indicated that late-onset cohort (GG versus TT : OR = 0.643, 95% CI = 0.420–0.985, p=0.042), male cohort (TG versus TT : OR = 2.160, 95% CI = 1.162–4.016, p=0.015), and female group (GG versus TT : OR = 0.418, 95% CI = 0.234–0.746, p=0.003) of rs1983474 were significantly associated with Parkinson's disease susceptibility. In both genotype and subgroup analysis, we failed to find any relationship between rs12681349 polymorphism and Parkinson's disease risk. Our results indicate that the rs10503253 and rs1983474 gene polymorphism may be associated with idiopathic Parkinson's disease susceptibility in Chinese population. Nevertheless, these conclusions need to be further verified by more studies.

Highlights

Parkinson’s disease (PD) is a common progressive neurodegenerative disease second only to Alzheimer’s disease, affecting about 1-2% of people over the age of 65 years [1, 2].e main clinical symptoms of PD are quiescent tremor, bradykinesia, ankyloses, and postural instability
receiver operating curve (ROC) showed that rs1983474 and rs10503253 have a low diagnostic ability in Parkinson’s disease
The relationship between CSMD1 single nucleotide polymorphisms (SNPs) and PD has not been explored so far. ree selected SNPs of CSMD1 gene were the protagonists of this study, including rs10503253, rs12681349, and rs1983474. e rs10503253 SNP was reported as an important genome-wide mutation in schizophrenia by Schizophrenia Psychiatric Genome-Wide Association Study (GWAS) Consortium [24]. e risk “A”

Summary

Introduction

Parkinson’s disease (PD) is a common progressive neurodegenerative disease second only to Alzheimer’s disease, affecting about 1-2% of people over the age of 65 years [1, 2].e main clinical symptoms of PD are quiescent tremor, bradykinesia, ankyloses, and postural instability. Nonmotor symptoms of PD are getting more and more attention, including cognitive impairment, depression, anxiety, and autonomic nerve dysfunction, and olfaction disorder [3, 4]. Females are less likely to develop PD and studies have found that estrogen in females can reduce the dysfunction of dopaminergic neurons in PD patients [5]. The etiology and pathogenesis of Parkinson’s disease are unclear. Beyond the mutations of candidate genes, the interaction between susceptibility genes and environmental factors plays an important role in the complex etiology of PD. Genome-wide association study (GWAS) has contributed to the discovery of genes associated with PD and other lesser-known pathways, including inflammation and immune dysfunction, dysregulation, transcription, vascular pathology, and neurotransmitter [6]

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