Abstract
A novel method for detecting Cu2 +ion was developed based on the fluorescence quenching effect of copper ion on carbon dots( CDs). The fluorescent probe was prepared by grafting reduced carbon dots( rCDs) in calcium alginate to form a calcium alginate film( CA-rCDs). Fluorescence characteristics of the probe and interaction between the probe and metal ions were studied using fluorescence spectrophotometer and UV-Vis spectrophotometer. The results show that the fluorescence intensity of the novel probe is strong and varied with the concentration of Cu2 +. Moreover, the quenched fluorescence intensity of probe is linearly proportional to the concentration of Cu2 +in the range from 5 × 10- 6to 100 × 10- 6mol·L- 1. In addition,it is found that the probe can detect Cu2 +repeatedly by using EDTA. This method is not only suitable for the detection of Cu2 +,but also can realize the immobilization of CDs,ensuring the probe to be recycled and reused.
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