叶绿素荧光技术对受Cu2+胁迫藻类暗适应时间的研究

Abstract

The best dark adaptation time of Chlorella pyrenoidosa,Snedesmus obliquus and Microcystis aeruginosa stressed by Cu2+ was investigated by chlorophyll fluorescence measurements.Photosynthetic fluorescence parameters were measured under light and dark conditions.Measurement time in light condition were 30s,1min,3min,5min,10min and 20min as well as in dark condition.Significant difference of dark adaptation time was analyzed mainly based on the photochemical quenching parameter and t-test method.The results show that compared to light,the potential maximum quantum efficiency values of three algaes increase slightly under dark adaptation and the yield value remaines the same;the photochemical quenching parameter value and non photochemical quenching parameter value increase significantly as time goes on;the maximum photochemical quenching parameter value of Microcystis aeruginosa achieves when the algae is treated under light in 1min,and Microcystis aeruginosa needs no dark adaptation may because blue-green algae state conditions shifts under dark adaptation;the dark adaptation time of algaes are not better by long time treated,and the best dark adaptation time of Chlorellapyrenoidosa and Snedesmus obliquus are 5 min and 10 min respectively.All of these will provide a reliable basis for further studies on the inhibiting effect of toxicant on algaes.