Abstract
Rhizobacteria may enhance biomass production and heavy metal tolerance of plants under stress conditions. The present study was carried out for isolation of metal-resistant bacteria that can be further utilized for phytoremediation process. A potential metal-resistant strain CRB15 was isolated from rhizospheric region of Saccharum spontaneum that was found to be resistant against Cu (6.29 mM), Zn (3.25 mM), Pb (1.5 mM), Ni (1.25 mM), and Cd (0.25 mM). SEM analysis was performed for evaluation of morphological changes on bacterial isolate. FTIR analysis observed the change in wavenumbers after the addition of Cu. 16S rDNA sequence analysis showed that CRB15 isolate matched best with genus of Kocuria and was named as Kocuria sp. CRB15. The isolate Kocuria sp. CRB15 was a potential plant growth-promoting rhizobacterium as it had a high IAA (46 µg ml−1), P solubilisation (39.37 µg ml−1), ammonia production (30.46 µmol ml−1), and hydrogen cyanide production capacity. Root–shoot elongation assay conducted on Brassica nigra under lab conditions with strain CRB15 demonstrated positive effects of strain CRB15 in root and shoot elongation of Cu-treated seedlings. This study proved the Kocuria sp. CRB15 a potential PGPR for bacterial-assisted phytoremediation.
Highlights
Copper (Cu) tailings, produced from mining, smelting, and processing of copper ores, may cause severe destruction to ecosystem including plants, animals, microbes, and human health (Wong 2003)
FTIR analysis observed the change in wavenumbers after the addition of Cu. 16S rDNA sequence analysis showed that CRB15 isolate matched best with genus of Kocuria and was named as Kocuria sp
CRB15 isolated from abandoned Cu tailing was evaluated for its heavy metal resistance and plant growth-promoting activities
Summary
Copper (Cu) tailings, produced from mining, smelting, and processing of copper ores, may cause severe destruction to ecosystem including plants, animals, microbes, and human health (Wong 2003). Cu is an essential micronutrient; its elevated level may affect the microbially mediated processes. It is essential to remove the Cu pollution from the environment. Remediation of polluted sites using the conventional techniques, such as physico-chemical extraction, land filling, soil washing, and excavation, is costly with high energy and chemical consumption (Jeyasingh and Philip 2005). An effective biotechnological approach ‘‘Bioremediation’’ has received increasing attention as an alternative to clean up a polluted environment due to its cost-effective and ecofriendly nature (Wu et al 2006). To have a crucial role in remediation, microorganisms in heavy metal-contaminated environment must first cope up with the heavy metal stress by means of immobilization or transformation (Ma et al 2011)
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