Cu(II)-Coordination Polymer Nanostructures Induce Human Lung Cancer Cell Carcinoma Apoptotic Cell Death In Vitro and In Vivo

Abstract

In the research, two novel coordination polymers based on Cu(II) ion as the metal source and their nanostructures have been prepared via utilizing two ligands of positional isomeric pyridine carboxylic acid 2-(5-carboxypyridin-3-yl)terephthalic acid (H3L2) and 4-(6-carboxypyridin-3-yl)phthalic acid (H3L1) under the conditions of ultrasonic and solvothermal reaction, and their chemical formulas are {[Cu3(L1)2(H2O)6](H2O)7}n (1) along with {[Cu3(L1)2(H2O)2](H2O)2}n (2). In addition, the treatment effect of 1 and 2 on the human cancer in vitro and in vivo was detected. The Cell Counting Kit-8 (CCK8) method was used to determine the proliferation of human lung cancer cell line (NCI-H292) after nanoparticles 1–2 treatment, the results exhibited that nanoparticle 1 had excellent inhibitory function on NCI-H292 cell viability. Besides, ROS accumulation in the NCI-H292 cells was detected by reactive oxygen species (ROS) assay and annexin V-FITC/PI assay was conducted to detect the apoptotic cells numbers of NCI-H292 cells induced by nanoparticles 1–2 treatment. In addition, the tumor model was established in vivo and the treatment ability of the nanoparticle on NCI-H292 tumor was evaluated by measuring the mice weight and tumor volume.