Abstract
C-type lectins (CTLs) play important roles in the innate immunity of invertebrates. In this study, a novel CTL with a single carbohydrate recognition domain (CRD) containing an EPN (Glu-Pro-Asn) motif was identified from the giant freshwater prawn Macrobrachium rosenbergii. This CTL was designated as MrCTL. The cDNA of MrCTL is 1788 bp with a 657 bp open reading frame that encodes a protein of 218 amino acids. The cDNA and genome sequences of MrCTL show a polymorphism that leads to MrCTL isoforms. MrCTL was highly expressed in the gills and intestine of normal prawn, and its transcription increased after Vibrio parahaemolyticus or white spot syndrome virus (WSSV) challenge. Recombinant mature MrCTL and its single CRD could agglutinate (Ca2+-dependent) and bind both Gram-positive and Gram-negative bacteria. The recombinant proteins could attach to lipopolysaccharide and peptidoglycan in a dose-dependent manner. Recombinant MrCTL could accelerate bacterial clearance. Thus, MrCTL could serve as a pattern recognition receptor involved in the innate immunity of M. rosenbergii.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
